Supplementary Figure 5: Transient complementation assays in Nicotiana benthamiana with six Rpi-amr3 candidates
From: Accelerated cloning of a potato late blight–resistance gene using RenSeq and SMRT sequencing
Third leaves of N. benthamiana plants were infiltrated with the binary vector pICSLUS0003::35S overexpressing either the late blight resistance gene R2 (positive control), six Rpi-amr3 candidates, Rpi-amr3i-S cloned from the susceptible accession 2271 or GFP (negative control). Leaves are 24 hours later inoculated with P. infestans strain 88069. Only leaves infiltrated with R2 and Rpi-amr3i remained infection free, while P. infestans was able to proliferate on the remaining Rpi-amr3 candidates, the susceptible allele of Rpi-amr3i-S as well as the GFP control. Scale bar indicates 1cm. These experiments were repeated twice with similar results.
