Supplementary Figure 8: Growth of xenografted glioblastoma cells is not impaired in mice fed diets devoid of one EAA.

(a) 2x10⁶ Gli36-luc cells were implanted subcutaneously in nude mice. A week after, mice were divided into two groups: one fed a control diet and a second group fed with an alternation of EAA-deficient diets as described in figure 2C. For tumor growth monitoring, mice bearing tumors were imaged on the first day after Gli36-luc implantation (T0) and subsequently every week. Signal intensity within the region of interest was quantified using ROIs covering the tumors. Photon detection is graded from red (highest number of photons) to blue (lowest intensity). No significant difference was observed for radiance, between the 2 groups along the time. (b) Tumors weight analysis. At the end of the experiment, excised tumors were photographed and weighted. No significant difference was observed for tumor weight, between the 2 groups along the time. (c) Nutritional control of the GCN2-eIF2α-ATF4 pathway. At the end of the experiment, tumors from the Ctrl group fed a complete diet (Ctrl/Ctrl) were excised at day 21. Within the -EAA group, half of the lot was sacrificed at day 21 whereas the other half was killed a day later. For more details see legend of Figure 4. Proteins homogenates from the excised tumors were analyzed by western blot for detection of P-eIF2α and ATF4 levels. Actin levels are shown as a loading control.