Supplementary Figure 2: GCN2 requirement for the AARE-Driven Expression System inducibility by leucine starvation. | Nature Biotechnology

Supplementary Figure 2: GCN2 requirement for the AARE-Driven Expression System inducibility by leucine starvation.

From: Regulating the expression of therapeutic transgenes by controlled intake of dietary essential amino acids

Supplementary Figure 2

(a) Wild type (WT), GCN2 -/-, PERK -/- or PKR -/- MEFs were transiently transfected with the construction described above. Twenty-four hours after transfection, cells were leucine-starved for 16 h and assayed for luciferase activity (Student's t-test: ***p <0.001, n=9; error bars: means ± s.e.m.). (b) Leucine concentration response of the AARE-Gene system inducibility. MEFs were transiently transfected with the AARE-Luc construction. Twenty-four hours after, cells were incubated for 16 h in media containing decreasing concentrations of leucine and assayed for luciferase activity (Student's t-test: ***p <0.001 in comparison to the control condition (420 μM), n=9; error bars: means ± s.e.m.).

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