Supplementary Figure 5: Assessment by bioluminescence imaging of the lentivirus dissemination in the mouse body following pancreatic transduction with LV-AARE-Luc.

(a) Administration of lentiviral particles containing the AARE-TK-Luc was performed into the pancreas of wild type mice. Ten days after injection, mice were challenged with the nutritional protocol (-Ile diet) for 6h and bioluminescence was measured. After pancreas and the adjacent spleen have been collected, organs and the body were imaged for bioluminescence. (b) PCR amplification for genotyping of the spleen and pancreas collected in Figure Supplementary 5a (primers used for PCR: forward primer, 5′-TCTGGTGGCGCTCCCCTCTC-3′; reverse primer, 5′-AACAACAACGGCGGCGGGAA-3′).