Supplementary Figure 3: Western blots of protein extracts from homozygous transgenic T2 canola seed expressing algal PUFA synthase
(a) PUFA synthase western blots. A single SDS-PAGE gel was loaded, run then cut as indicated by the dotted lines. The left-hand section was processed with anti-PFA1 antibody, the center panel with anti-PFA2 antibody and the right-hand panel with anti-PFA3 antibody. An appropriate purified protein standard was run adjacent to each seed extract. Little evidence for proteolytic degradation was noted for PFA2 and PFA3. The major PFA1 band accounts for 75% of the signal intensity in the transgenic seed extracts. (b) NoHetI western blot. The slightly larger size of the PFA2, PFA3 and NoHetI standards and the proteins expressed in plants is due to the 6xHis-tag on the standards.
