Supplementary Figure 7: Quantification of APC and KRASG12D expression in hCECs.
From: A recellularized human colon model identifies cancer driver genes
The psi-LVRH1GP (CMV-H1-APC shRNA-SV40-KRASG12D - neomycin) lentivirus, containing both an APC shRNA and a human KRASG12D expression construct, was transduced into hCEC cells. Protein expression levels of APC and a Myc-DDK tag for KRASG12D in hCEC cells were quantified by Western blotting (+). hCEC cells transduced with the backbone vector served used the control (-). β-actin is loading control.
