Supplementary Figure 8: Tumor formation in mice injected with APC-KRASG12D hCECs.
From: A recellularized human colon model identifies cancer driver genes
2x106 matrigel-embedded parental or APC-KRASG12D hCECs were subcutaneously injected into each flank of 6-8 week old NSG mice (NOD/Shi-scid/IL-2Rγnull mice). The TGF-β signaling pathway was then blocked through the intraperitoneal injection of a TGF-β receptor inhibitor (TGF-β RI Inhibitor III), beginning at two weeks after injection (100mg/kg) with continuing injections three times per week. Six weeks after injection of APC-KRASG12D hCECs, tumor nodules (>250mm3) were visible at 60-80% of the injection sites, while injection of parental hCECs did not induce any tumors. (A) A representative tumor is shown following H+E staining. Scale bars, 100µ. (B) Quantification of tumor formation following injection of APC-KRASG12D hCECs and parental hCECs. ** P=0.0002, comparison in tumor formation by Fisher’s exact test.
