Supplementary Figure 9: Creation of a modified SB transposition system for use in human tissues.
From: A recellularized human colon model identifies cancer driver genes
(A) Transposition activity was quantified using a fixed dose of transposon-donor plasmid (500 ng T2/Onc1) and varying doses of SB100X transposase plasmid; error bars indicate S.E.M. The activity of transposase reached its peak at a dose of 100ng. n = 5 independent experiments. (B) Average transposon copy numbers in individual hCEC colonies.
