Supplementary Figure 2: The procedure of recellularization.
From: A recellularized human colon model identifies cancer driver genes
The acellular colon matrix was physically separated into (A) mucosal (20x and 40x in big and small windows, respectively) and (B) submucosal layers. White arrow highlights intact epithelial crypt niches in the mucosal layer. Scale bars, 1mm. (C) Endothelial cells are shown being loaded into an injection needle and (D) being injected into the mucosa layer. Scale bars, 200 µ. The acellular colon matrix (E) was populated with colonic epithelial cells, endothelial cells and myofibroblasts. (F) A colon after recellularization for 6 weeks. Scale bars, 2 mm. (See Supplementary Video 1, 2 and 3 for detailed procedures.)
