Supplementary Figure 5: Molecular characterization of physiologically active crypts recellularized with hCECs.

Immunostaining of the recellularized crypts showed that they contain cells (yellow denoted by white arrows) double positive for the intestinal stem cell marker Lgr5 (red) and proliferative marker Ki67 (green) (top panel) in addition to differentiated colon epithelial cells (Muc1 positive cells, transit amplifying cells), goblet cells (Muc2 positive cells), and enteroendocrine cells (chromogranin A-positive cells) (bottom panels). All the recellularized tissues had been cultured for 4 weeks before they were terminated from culture and prepared for immunostaining. Blue nuclei, DAPI; Scale bars, 20 µM. n= 3 independent cultures.