Supplementary Figure 7: Evidence of primer editing and differential recovery of multiple taxa between the EMP (Taq) and DI (KAPA) methods in human samples.
From: Systematic improvement of amplicon marker gene methods for increased accuracy in microbiome studies
A) Percent abundance of the k__Bacteria;p__TM7;c__TM7-3;o__CW040;f__F16;g__ taxon as measured by either the EMP (Taq) or DI (KAPA) method.
B) Logo plots and alignments of V4 515F and V4 806R primer sequences to the corresponding region in reads assigned to the k__Bacteria;p__TM7;c__TM7-3;o__CW040;f__F16;g__ taxon in the 7013.02.CF sample. Position with mismatch to the V4 515F primer is highlighted in red.
C) Percent abundance of the k__Bacteria;p__TM7;c__TM7-3;o__;f__;g__ taxon as measured by either the EMP (Taq) or DI (KAPA) method.
D) Logo plots and alignments of V4 515F and V4 806R primer sequences to the corresponding region in reads assigned to the k__Bacteria;p__TM7;c__TM7-3;o__;f__;g__ taxon in the 7000.01.CF sample. Position with mismatch to the V4 515F primer is highlighted in red.
E) Percent abundance of the k__Bacteria;p__Actinobacteria;c__Actinobacteria;o__Actinomycetales;f__Propionibacteriaceae;g__Propionibacterium taxon as measured by either the EMP (Taq) or DI (KAPA) method.
F) Logo plots and alignments of V4 515F and V4 806R primer sequences to the corresponding region in reads assigned to the k__Bacteria;p__Actinobacteria;c__Actinobacteria;o__Actinomycetales;f__Propionibacteriaceae;g__Propionibacterium taxon in the 6998.01.CF sample. Positions with mismatch to the V4 515F and V4 806R primers are highlighted in red.
