Supplementary Figure 11: Crossing red GPR-1 OE hermaphrodites with green males resulted in two types of cross-progeny: F1 that co-expressed both markers, and F1 that expressed the markers in separate tissues.
From: Engineered non-Mendelian inheritance of entire parental genomes in C. elegans
(A) Background lethality was measured for wild type and the different strains that were used for crossing. The two marker strains showed lethality in the control genetic background in which the crosses were made. GPR-1 overexpression led to an increased lethality of 27.8%(450/1616) (p < 0.001 when comparing oxTi411 with oxTi411; ddIs32). In the GPR-1 OE strain, mating with males further increased lethality to 63.1% (748/1186) (p < 0.001, compared with lethality in offspring resulting from self-fertilization). Increased lethality after mating may be due to a lack of the X chromosome, but other causes such as imprinting or strain differences may contribute to lethality as well. 40 dead embryos were checked for marker expression. From this sample, 80% co-expressed the markers and 20% expressed them separately, indicating that 80% of the dead embryos were derived from a zygote that unequally segregated the genomes.
(B) Crossing of the two marker strains in the absence of GPR-1 OE resulted exclusively in F1 cross progeny that were co-expressing both markers in all cells. For each cross, one red hermaphrodite was crossed with three green males.
(C) Crossing both markers combined with GPR-1 OE resulted not only in F1 cross progeny that were co-expressing both markers in all cells, but also in F1 cross progeny that were expressing the markers in separate tissues. For each cross, one GPR-1 OE hermaphrodite was crossed with three green males. The efficiency with which worms with separate marker expression were formed among the surviving cross progeny varied between 18 and 77 %, and separate marker expression was never observed in the control crossings [N = 331 (total number of surviving offspring resulting from all crosses in the presence of GRP-1 OE), N = 1781 (total number of surviving offspring resulting from all control crosses in the absence of GPR-1 OE), p < 0.001].
