Supplementary Figure 5: Despite forming two monopolar spindles in the P0 zygote, embryos formed normal bipolar spindles in subsequent divisions.
From: Engineered non-Mendelian inheritance of entire parental genomes in C. elegans
Left top panel shows a DIC image of an embryo during P0 metaphase. Two monopolar spindles have formed, each from either the paternal (P) or maternal (M) pronucleus (magenta arrowheads). Right top panel shows a fluorescent image of YFP::GPR-1. The spindle poles are indicated with green arrowheads. Bottom left panel shows the same embryo 20 minutes later, during the second round of cell division. Shown is the time of P1 metaphase. The P1 metaphase plate that is derived only from paternal DNA is indicated with a magenta arrowhead. Right bottom panel shows YFP fluorescence with spindle poles indicated by green arrowheads. Scale bar is 50 ÎĽm.
