Supplementary Figure 11: Culturing AML cells ex vivo.
From: High-throughput measurement of single-cell growth rates using serial microfluidic mass sensor arrays
We seeded cells obtained from Patient 1 (Figure 4 and Supplementary Figure 10) at 0.5, 0.7 and 1.5 million cells/mL (blue, red, and black, respectively) into 6-well culture dishes. (A) We profiled these cultures volume distributions over the next 48 hours with a coulter counter (Beckman Coulter Multisizer 4, 100 μm aperture). The cultures behaved similarly for different inoculation densities. (B) Total cell counts generally increased slightly, but total biovolume decreased.
