Supplementary Figure 10: Molecular perturbations of yeast lacking yjr120w.
From: Mitochondrial protein functions elucidated by multi-omic mass spectrometry profiling
(a) Relative protein abundances (mean log2[Δyjr120w/WT], n = 3) versus statistical significance (–log10[p-value]; two-sided Student’s t-test) as quantified by MS. (b) Relative Atp2p protein abundance (mean log2[Δgene/WT], n = 3) versus statistical significance (–log10[p-value]; two-sided Student’s t-test) across all mutants in the study. (c) Genomic organization of yjr120w and atp2. (d) Serial dilutions of yeast transformed with the indicated plasmids grown on agar plates with glucose (to enable fermentation) or glycerol (to force respiration). (e) Fold changes in mRNA abundances (mean Δgene/WT, n = 3) as quantified by real time polymerase chain reaction (RT-PCR) analysis. Yjr120w mRNA was not detected (n.d.) in WT yeast, so imputation of this missing value was used to calculate the fold increase in yjr120w mRNA shown for the Δatp2 strain. *P < 0.05; **P < 0.01; ***P < 0.001 (two-sided Student’s t-test).
