Supplementary Figure 4: RA signaling does not affect the efficiency of SANLPC development. | Nature Biotechnology

Supplementary Figure 4: RA signaling does not affect the efficiency of SANLPC development.

From: Sinoatrial node cardiomyocytes derived from human pluripotent cells function as a biological pacemaker

Supplementary Figure 4

(a) Individual data point dot plots of the flow cytometric analyses presented as stacked bar graph in Fig. 2e showing the proportion of NKX2-5+cTNT+ and NKX2-5-cTNT+ cells at day 20 of differentiation generated from mesoderm (3 ng/ml BMP4, 2 ng/ml ACTA) treated with retinoic acid (RA) on days 2-12 (n = 4). (b) Individual data point dot plots of the flow cytometric analyses presented as stacked bar graph in Fig. 2g showing the proportion of NKX2-5+cTNT+ and NKX2-5-cTNT+ cells at day 20 of differentiation generated from mesoderm (3 ng/ml BMP4, 2 ng/ml ACTA) treated with BMP4 (2.5 ng/ml) together with SB (5.4 μM) and/or different concentrations of retinoic acid (RA) between days 3-5. t-test: **P < 0.01 vs Nkx2-5-cTNT+ cells in untreated control condition (n = 4). All error bars represent s.e.m. d, day

Back to article page