Supplementary Figure 1: Characterization of PBMC-derived iPSCs

A) Graph shows no correlation (Pearson r -0.22) between the number of chromosomal abnormalities in PBMC-derived iPSCs and the age of the donor. B) Reprogramming efficiency did not correlate with the age of the donor. C) Table showing the characterization of all iPSC lines used in the study. iPSC lines were tested for karyotyping and pluripotency markers, TRA 1-60 and SSEA4, by cytofluorimetric analysis. Table shows the lines used for epigenetic analysis, as well as, the ones used for somatic mutation calls. Passage for each assay is shown. Abbreviations: HTN: Hypertension; CAD: Coronary Artery Disease; MI: Myocardial infarction; CA: Cancer; PAD: Peripheral Artery Disease; GERD: Gastroesophageal reflux disease; SVT: Supraventricular tachycardia. Blood donors were identified either through participation in the Normal Blood Donor service at TSRI (a walk in blood donation program), or through participation in other studies ongoing in the Topol laboratory including the Geneheart (individuals diagnosed with coronary artery disease) and Wellderly (individuals greater than 80 years old with no common chronic conditions) cohorts. While the majority of blood donors appeared to be normal and healthy, some donors had been diagnosed with common aging associated diseases reflective of those seen in the general population. None of these conditions are associated with genome instability in blood. While it is possible that rare donors could have undiagnosed conditions that could impact the number of mutations in blood cells, these are expected to be very rare and due to the large number of donors and cell lines we sampled, such events would not impact overall trends we report. Some of the iPSC lines have been banked and are available at WiCell, in a collection called Topol Lab’s Next Gen Cell Lines. The cell line numbers in Table C correspond to the following accession numbers in WiCell: Young: NBD5332#3 (SCRP4203i), NBD5396#3 (SCRP4403i), NBD5433#5 (SCRP4505i), NBD5532#2 (SCRP4602i), NBD5361#5 (SCRP4305i). Middle: C087#2 (SCRP0202i), C512#17 (SCRP0517i), C151#7 (SCRP0307i), C568#1 (SCRP0601i), C664#9 (SCRP0709i). Elderly: HE463#5 (SCRP2305i), HE463#7 (SCRP2307i), HE463#10 (SCRP2310i), HE787#3 (SCRP2503i), HE787#6 (SCRP2506i), HE787#8 (SCRP2508i), C939#2 (SCRP0802i), HE019#1 (SCRP2101i), HE019#6 (SCRP2106i), HE019#15 (SCRP2115i), HE157#8 (SCRP2208i), HE157#10 (SCRP2210i), HE157#11 (SCRP2211i), HE554#7 (SCRP2407i), HE554#9 (SCRP2409i), HE554#11 (SCRP2411i).