Supplementary Figure 4: Rapid cortical neuronal induction in hiPSC lines. | Nature Biotechnology

Supplementary Figure 4: Rapid cortical neuronal induction in hiPSC lines.

From: Combined small-molecule inhibition accelerates the derivation of functional cortical neurons from human pluripotent stem cells

Supplementary Figure 4

(a) Validation of P1S5D and (b) P8S10D protocols on hiPSC lines by immunocytochemistry of TBR1/TUJ1 expression at day 13. (c) Quantification of neuronal induction efficiency at day 13 by intracellular flow cytometry for various hiPSC lines tested. N = 3 independent batches of cell cultures for line 1.1, 1.2, 1.3, and N = 1 for line 7.1, 7.2 and 7.4. Scale bars: 50 μm. Error bars represent s. e. m.

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