Supplementary Figure 5: Molecular characterization of long-term culture beyond day 13. | Nature Biotechnology

Supplementary Figure 5: Molecular characterization of long-term culture beyond day 13.

From: Combined small-molecule inhibition accelerates the derivation of functional cortical neurons from human pluripotent stem cells

Supplementary Figure 5

(a) Quantification of mRNAs expression in P1S5D and P8S10D treated culture (Fig. 3a) compared to LSB+X treated cultures. RGS4: cortical layer II-III,V marker. CHX10: retinal marker. GFAP, AQP4: astrocyte marker. OLIG2: oligodendrocyte precursor marker. For long-term culture of LSB+X cells, cells were maintained in N2 medium without re-adding small-molecule inhibitors. N = 3 independent batches of cell cultures. Error bars represent s. e. m.

Back to article page