Supplementary Figure 9: iDISCO based whole brain immunofluorescence analyses of P8S10D and LSB+XAV grafts at 1 month after transplantation.

(a) P8S10D grafted half brain, stained for GFP (whole view and details of the frontal cortical region). P8S10D grafts showed inconsistent survival after transplantation into neonatal mouse cortex. However, animals with surviving graft showed long fiber projections across cortical regions. GFP+ cells devoid of axons were abundantly detected outside of the graft (boxed region). (b) LSB+X grafted half brain, stained for GFP. Whole view (side and dorsal) and detail of the graft margin (boxed region). LSB+X grafts showed massive overgrowth in host brain resulting in tumor-like structures with very limited evidence of neuronal differentiation and maturation. Only a few short fiber tracts can be seen at the margin of the graft (boxed region). N = 2 animals for P8S10D condition, and 4 animals for LSB+XAV condition analyzed. Scale bars: 500 μm.