Supplementary Figure 2: Gating of flow cytometry and impact of various small-molecule manipulations on FOXG1 expression. | Nature Biotechnology

Supplementary Figure 2: Gating of flow cytometry and impact of various small-molecule manipulations on FOXG1 expression.

From: Combined small-molecule inhibition accelerates the derivation of functional cortical neurons from human pluripotent stem cells

Supplementary Figure 2

(a) Gating of intracellular flow cytometry of TUJ1+ neuron population at day 13 for the various culture protocols used (blue: TUJ1 stain. red: isotype control). (b) Immunocytochemistry for FOXG1/TUJ1 co-expression at day 13. (c) Quantification of FOXG1 transcript expression level by qRT-PCR under P1S5D conditions but after systematic removal of one of the small molecules each. N = 3 independent batches of cell cultures. Scale bars: 100 μm. Error bars represent s. e. m.

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