Supplementary Figure 3: Characterization of additional fate markers at day 13 of differentiation. | Nature Biotechnology

Supplementary Figure 3: Characterization of additional fate markers at day 13 of differentiation.

From: Combined small-molecule inhibition accelerates the derivation of functional cortical neurons from human pluripotent stem cells

Supplementary Figure 3

(a) Expression of layer VI marker TLE4 in post-mitotic neurons, and CUX2 expression in progenitors at day 13 of differentiation in both P1S5D and P8S10D induction. (b) Quantification of mRNA expression at day 13 of markers other than TBR1 representing different brain areas and fates. N = 3 independent batches of cell cultures. Cortical progenitor marker OTX2, ZNF521, BRN2 and COUPTF1, layer V cortical neuron marker CTIP2, and the vesicular glutamate transporter VGLUT1 are upregulated compared to LSB+X. (c) Expression of BRN3A+ and ISL1+ cells in both P1S5D and P8S10D cultures at day 13. (d) Quantification of BRN3A+ cells at day 13. Scale bars: 50 μm. Error bars represent s. e. m.

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