Supplementary Figure 1: Output of GuideScan and competitor tools.
From: GuideScan software for improved single and paired CRISPR guide RNA design
(a) Genome-wide density of guides in GuideScan’s murine Cas9 database (blue), compared to the UCSC genome track from mit.edu (red). (b) Dot plot showing specificity scores and number of perfect off-target sites for promiscuous guides designed by the mit.edu gRNA web design tool. Data points are color-coded based on the specificity scores of the corresponding gRNAs following the guidelines of mit.edu web portal (red = low specificity, score 1-19; yellow = medium specificity, score 20-49; green = high specificity, score 50-100).
