Supplementary Figure 4: MCS Lck only was insensitive to ionic strength change.

MCS Lck only is MCS⁺ without the electrostatic membrane attachment unit (MA2 in Fig. 1). (a) Merged donor (green) and acceptor (red) intensity images of MCS Lck only in membrane sheets before and after solution change from 10 mM to 275 mM. Scale bar = 5 μm. (b) Donor (green) and acceptor (red) and acceptor/donor (R/G) ratio before and after solution change (black arrow) from 10 mM to 275 mM. MCS Lck only was insensitive to the effective electrostatic potential as the R/G ratio changed by < 5%. Please note that MCS Lck only remained attached to the membrane lawn indicated that the hydrophobic anchor (MA1 in Fig. 1) was sufficient for membrane attachment. Data are representative of n = 7 independent experiments.