Supplementary Figure 10: MCS Lck only and MCS Hras FI were largely unresponsive to calcium fluxes induced by thapsigargin and hypotonic and hypertonic treatments.
From: A FRET sensor enables quantitative measurements of membrane charges in live cells
FRET efficiency of MCS Lck only (a) and MCS Hras FI (b) in COS-7 cells before (black symbols) and after (red symbols) thapsigargin, hypotonic (150 mM to 40 mM) and hypertonic (150 mM to 450 mM) treatments. FRET efficiencies were normalized to the mean of untreated cells. Each symbol is one measurement in one cell; horizontal and vertical bars represent mean and standard error, respectively. ns, not significant (P > 0.05); * P < 0.05; ** P < 0.01 (unpaired, two-tailed t-test).
