Supplementary Figure 12: FRET efficiency of MCS Lck only and MCS Hras FI in response to fendiline and wortmannin treatments.
From: A FRET sensor enables quantitative measurements of membrane charges in live cells
(a-b) FRET efficiency of MCS Lck only (a) and MCS Hras FI (b) in COS-7 cells before (black symbols) and after (red symbols) fendiline (60 μM) and wortmannin (10 μM) treatments in fixed cells. FRET efficiencies were normalized to the mean of untreated cells. Each symbol is one cell of n = 30 independent measurements; horizontal and vertical bars represent mean and standard error, respectively. ns, not significant (P > 0.05); * P < 0.05 (unpaired, two-tailed t-test).
