Supplementary Figure 4: Long-term stability of PAS638 during colonization of the mouse gut.

a) Essentially all PAS638 colonies tested following 200 days colonized in the 129X1/SvJ mouse (corresponding to Fig. 3e-f) retained ex vivo function when grown in the presence of sodium tetrathionate under anaerobic conditions. PAS638 colonies isolated after 200 days colonization were administered to C57Bl/6 mice ± S. typhimurium (S.tm) ΔttrR. b) Histology scoring of cecum and colon using a 0-4 point scale documented in Supplementary Fig. 2f detected elevated signs of inflammation in S. typhimurium ΔttrR co-infected mice. c) S. typhimurium ΔttrR infected mice also lost weight following infection (graph shows single mouse values (dotted lines) as percentage of pre-administration weight along with group averages (solid lines)) and d) showed elevated LCN-2 values. Graph shows maximum values measured from d1-5 post bacterial administration. ****p<0.0001 using separate two-tailed T-tests for the two individual experiments (t(8) = 16.55 and t(8) = 19.38 respectively). Means are shown.