Supplementary Figure 5: Heat treatment significantly improves immunostaining of vimentin and actinin-4 on human kidney samples.
From: Nanoscale imaging of clinical specimens using pathology-optimized expansion microscopy
Left panel, widefield fluorescent images of acetone fixed human kidney sections with and without heat treatment in citrate buffer. Right panel, zooms into regions corresponding to the regions indicated by the dashed yellow rectangles in the left panels. Scale bars: 1 mm (left panel), 200 μm (right panel). Abbreviations: ACTN4, actinin-4; ColIV, collagen IV.
