Supplementary Figure 6: Anti-actinin-4 specifically stains tertiary podocyte foot processes.
From: Nanoscale imaging of clinical specimens using pathology-optimized expansion microscopy
(A-D) Post-expansion widefield images of a human acetone fixed kidney section stained with DAPI and antibodies. Blue, vimentin; green, actinin-4; red, synaptopodin; gray, DAPI. (E) Merged image of (A-D). (F and G) Magnified regions showing actinin-4 (F) and synaptopodin (G) zoomed into the white dashed squares of B and C. (H) Profiles of fluorescent intensity taken along the white dashed line cuts of F and G. Green, actinin-4; red, synaptopodin. Scale bars: 1 μm (4.5 μm physical size, expansion factor 4.5).
