Supplementary Figure 7: Immunostaining images of FFPE kidney samples.
From: Nanoscale imaging of clinical specimens using pathology-optimized expansion microscopy
A. Post-expansion widefield image of a FFPE normal human kidney sample treated with a citrate antigen retrieval method (20 mM sodium citrate, pH 8.0), with highlighted region (boxed line) magnified in panel B. C. Post-expansion widefield image of a FFPE normal human kidney sample treated with a Tris-EDTA antigen retrieval method (10 mM Tris base, 1 mM EDTA solution, 0.05% Tween 20, pH 9.0), with highlighted region (boxed line) magnified in panel D. E. Post-expansion confocal image of a FFPE normal human kidney sample treated with a citrate antigen retrieval method, with highlighted region (boxed line) magnified in F. G. Post-expansion confocal image of a FFPE human kidney sample with minimal change disease treated with a citrate antigen retrieval method, with highlighted region (boxed line) magnified in H. All the samples were stained with DAPI (gray) and antibodies against vimentin (blue), actinin-4 (green) and collagen IV (magenta). Scale bars: A, C, E and G, 40 μm (physical size). B, D, F, and H, 8 μm (physical size).
