Supplementary Figure 3: Distortion evaluation of post-expansion HeLa cells processed by 25 mM EDTA-assisted proteinase K digestion. | Nature Biotechnology

Supplementary Figure 3: Distortion evaluation of post-expansion HeLa cells processed by 25 mM EDTA-assisted proteinase K digestion.

From: Nanoscale imaging of clinical specimens using pathology-optimized expansion microscopy

Supplementary Figure 3

(A) Super-resolution structured illumination microscopy (SR-SIM) image of a HeLa cell stained with DAPI and an antibody against α-tubulin. Blue, DAPI; green, α-tubulin. (B) Post-expansion image of the same sample acquired with a spinning disk confocal microscope. (C and D) Root mean square (RMS) length measurement error as a function of measurement length for ExPath vs SIM images (blue solid line, mean of DAPI channel; green solid line, mean of α-tubulin channel; shaded area, standard deviation; n = 3 samples from separate cultures). Scale bars: (A) 4 μm, (B) 4 μm (for yellow scale bar, physical size post-expansion, 19.5 μm; expansion factor: 4.89).

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