Supplementary Figure 8: enCORs with ECM addition display organized cortical plate
From: Guided self-organization and cortical plate formation in human brain organoids
a. Bright field images of H9 60-day spheroids lacking dissolved ECM and enCORs with dissolved Matrigel showing a band of density (arrow) suggesting the presence of a dense cortical plate. b. Higher magnification of immunostaining shown in Figure 3d for Laminin, MAP2 and Ctip2 showing the radial orientation of neurons that have reached the cortical plate (brackets). c. Side-by-side comparison of CP (arrowheads, brackets) in 60-day H9, 60-day iPSC, and 70-day H1 organoids stained by H&E. d. H&E staining of 60-day H9 enCORs with dissolved Matrigel show a dense band consistent with CP, whereas enCORs instead treated from day 30 with the matrix metalloprotease inhibitor GM6001 show no signs of CP formation. e. H&E staining of 60-day H9 enCORs with dissolved Matrigel compared with dissolved laminin, dissolved laminin/entactin complex, or a mixture of laminin/entactin and collagen I. Note the presence of a dense CP band with Matrigel, while laminin alone or in combination with entactin and collagen I display no clear CP and only occasionally a faint band of cells with laminin/entactin. Scale bars: 200 μm in a., 100 μm in b., c., d., e.
