Supplementary Figure 2: Bioengineered brain organoids display consistent generation of neural ectoderm
From: Guided self-organization and cortical plate formation in human brain organoids
a. Bright field image of the intact braided PLGA fiber. b. Bright field image of isolated microfilaments. c. Hydrated microfilaments within a droplet of EB medium. d. Three examples of microfilament engineered H9 EBs at day 3 of the protocol. Note the elongated and sometimes complex arrangement. e. Bright field image of collagen-type fibers isolated from dried sea sponge used to generate EBs in Figure 1b. f. Bright field imaging of cellulose fibers used to generate EBs in Figure 1b. g. Bright field images of seven independent batches of microfilament organoids on the day of transfer to NI (day 10 for batches 4,6; day 11 for batches 1, 2, 3, 5; day 12 for batch 7), showing reduced variability in generation of polarized neuroectoderm (arrows). h. Mean ratio of EBs per enCOR batch (same as in g.) displaying at least some neuroepithelium, recognizable in bright field as optically clear superficial tissue with a clean border and evidence of radial cellular architecture. i. Quantification of the mean ratio of spheroids or enCORs displaying neuroepithelium. Error bars are S.E.M., note the increased variance in spheroids compared with enCORs. *P<0.05, Student's two-tailed t-test, n = 7 independent batches each. Scale bars: 100 μm in a., 250 μm in b., c., d., g., 500 μm in e., f.
