Supplementary Figure 4: Protein co-regulation analysis identifies substantially more known protein–protein associations than mRNA co-regulation analysis across a range mRNA of precision thresholds.

The analysis was done on identical numbers of gene products monitored in 36 cell lines. The RNAseq data published by Klinj et al.1 were used for mRNA-based co-regulation analysis. High confidence known protein-protein associations were extracted from the STRING database (score ≥ 0.700)2. The co-regulation analysis is described in the Supplementary Methods Section.
1. Klijn, C. et al. A comprehensive transcriptional portrait of human cancer cell lines. Nat Biotechnol 33, 306-312, doi:10.1038/nbt.3080 (2015).
2. Szklarczyk, D. et al. The STRING database in 2011: functional interaction networks of proteins, globally integrated and scored. Nucleic Acids Res 39, D561-568, doi:10.1093/nar/gkq973 (2011).