Supplementary Figure 8: Determine the activity of genome editing in lung and spleen of mice after CRISPR treatment.
From: Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing
C57BL/6 or FAHmut/mut mice were injected with lipid nanoparticles encapsulated with e-sgRNAs targeting Pcsk9, or Fah (for FAHmut/mut mice) or ROSA26 and Cas9 mRNA. Indels at a) Pcsk9, b) Fah and c) ROSA26 loci in total DNA from the lung and spleen were measured by T7EI assay. n = 3. Gel images were cropped. See full length gels with molecular weight standard in Supplementary information
