Supplementary Figure 9: Determine the indels at the Fah sgRNA's off-target site obtained from GUIDE-seq.
From: Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing
FAHmut/mut mice were injected with lipid nanoparticles encapsulated with e-sgRNA targeting Fah and Cas9 mRNA. Indels at off-target site 1 (OT1) concluded from GUIDE-seq were measured by T7EI assay. n = 4. Gel images were cropped. See full length gels with molecular weight standard in Supplementary information
