Supplementary Figure 3: In vivo delivery of chemical modified sgRNAs and Cas9 mRNA induced knockout of targeted gene.
From: Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing
C57BL/6 mice were i.v. injected with two e-sgRNAs targeting Pcsk9 (PCSK9-1 & 2) and Cas9 mRNA encapsulated in lipid nanoparticles (Fig. 4). PCR was performed using two pairs of primers. The size of uncut and deletion bands (red pairs) is indicated. Both primer pairs show similar level of deletion band. Gel images were cropped. See full length gels with molecular weight standard in Supplementary information
