Supplementary Figure 6: Maximum-likelihood phylogenetic tree showing coverage of the domain Archaea. | Nature Biotechnology

Supplementary Figure 6: Maximum-likelihood phylogenetic tree showing coverage of the domain Archaea.

From: Retrieval of a million high-quality, full-length microbial 16S and 18S rRNA gene sequences without primer bias

Supplementary Figure 6

Maximum-likelihood phylogenetic tree showing coverage of the domain Archaea. The tree includes all archaeal OTUs clustered at 97% generated in this study, their closest match in the Silva SSU NR99 v. 128 database and the reference set from the recent Tree of Life article (Hug et al., 2016). Hypervariable regions were masked with a 40% positional conservation filter, giving 1257 alignment positions, and the tree calculated using FastTree v. 2.1.3 S SE3 (Price et al., 2010). Clade names and clustering are based on the position of reference sequences. *Indicates clades that do not include a genome or pure culture reference sequence – being based on classification of reference sequences in the Silva v. 1.28 taxonomy. Reference sequences appear black whilst those generated in the current study are color coded based on their similarity to existing database sequences.

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