Supplementary Figure 7: GUIDE-seq on-target specificities of high-fidelity SpCas9 variants
From: A highly specific SpCas9 variant is identified by in vivo screening in yeast
Cleavage specificity, expressed by the percentage of on-target reads captured by GUIDE-seq (from Fig. 3a and Supplementary Fig. 5, 6) using wild-type SpCas9, SpCas9-HF1, eSpCas9(1.1) and evoCas9, in combination with sgRNAs targeting the VEGFA2, VEGFA3, EMX1, CCR5, CXCR4, FANCF2, PD1 and HEK site4 loci.
