Supplementary Figure 10: W90Y and R126E mutations in rat APOBEC1 (rA1) narrowed the base-editing window to 3 nt

(a) Schematic diagram illustrating the design of expression vectors of dCpf1-BE, dCpf1-BE-YE and dCpf1-BE-YEE. (b) The normalized ratios of major editing to minor editing induced by dCpf1-BE (purple) and dCpf1-BE-YE (magenta), setting the ones induced by dCpf1-BE as 100%. (c) The fractions of single and multiple C-to-T conversions induced by dCpf1-BE and dCpf1-BE-YE. (d) Statistical analysis showed that the fraction of single C-to-T conversion induced by dCpf1-BE-YE was significantly higher than that induced by dCpf1-BE. P values, one-tailed Student’s T test. The median and IQR are shown. n = 15 independent samples from 3 independent experiments. (e) The indel frequencies were determined at the indicated genomic loci from the 293FT cells transfected with dCpf1-BE (purple), dCpf1-BE-YE (magenta), dCpf1-BE-YEE (yellow) or non-transfected (gray). Asterisk denotes an unusually high basal indel frequency (or amplification, sequencing, alignment artifact) at the examined RUNX1 site in the non-transfected 293FT cells. (b, e) Means ± s.d. were from three independent experiments.