Supplementary Figure 11: The fusion of three copies of 2A-UGI sequences did not substantially affect editing efficiency and induced no detectable indel formation | Nature Biotechnology

Supplementary Figure 11: The fusion of three copies of 2A-UGI sequences did not substantially affect editing efficiency and induced no detectable indel formation

From: Base editing with a Cpf1–cytidine deaminase fusion

Supplementary Figure 11

(a) Schematic diagram illustrating the design of expression vectors of dCpf1-BE and dCpf1-eBE. (b) The base editing frequencies induced by dCpf1-BE (purple) and dCpf1-eBE (green) were determined at indicated positions in genomic DNA. (c) The indel frequencies were determined at the indicated genomic loci. The 293FT cells were either treated with dCpf1-BE (purple), dCpf1-eBE (green) or left non-transfected (gray) before deep sequencing. (d) Schematic diagram illustrating the design of expression vectors of dCpf1-BE-YE and dCpf1-eBE-YE. (e) The base editing frequencies induced by dCpf1-BE-YE (magenta) and dCpf1-eBE-YE (brown) were determined at the indicated positions in genomic DNA. (f) The indel frequencies were determined at the indicated genomic. The 293FT cells were either treated with dCpf1-BE-YE (magenta), dCpf1-eBE-YE (brown) or non-transfected (gray) before deep sequencing. Asterisk denotes an unusually high basal indel frequency (or amplification, sequencing, alignment artifact) at the examined RUNX1 site in the non-transfected 293FT cells. Means ± s.d. were from three independent experiments.

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