Supplementary Figure 1: dLbCpf1-BE0 but not dAsCpf1-BE0 induced C-to-T base editing in the episomal shuttle vector system | Nature Biotechnology

Supplementary Figure 1: dLbCpf1-BE0 but not dAsCpf1-BE0 induced C-to-T base editing in the episomal shuttle vector system

From: Base editing with a Cpf1–cytidine deaminase fusion

Supplementary Figure 1

(a) Schematic diagram illustrating the procedures to determine the base editing induced by dLbCpf1-BE0 or dAsCpf1-BE0 in episomal shuttle vectors. (b) Number of E. coli colonies containing mutated shuttle vectors that were induced by dAsCpf1-BE0 or dLbCpf1-BE0. (c) C-to-T editing frequencies were determined at the indicated cytosines. The cytosines were counted with the base proximal to the PAM setting as position 1. Frequencies were calculated from data in (b). Means ± s.d. were from three independent experiments.

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