Supplementary Figure 2: Effect of crRNA spacer length on editing efficiency | Nature Biotechnology

Supplementary Figure 2: Effect of crRNA spacer length on editing efficiency

From: Base editing with a Cpf1–cytidine deaminase fusion

Supplementary Figure 2

(a) Number of colonies containing mutated shuttle vectors that were induced by dCpf1-BE0 and crRNAs of different length. (b) C-to-T editing frequencies induced by dCpf1-BE0 and crRNAs of different length were determined at the indicated cytosines in episomal shuttle vectors. The crRNAs with the spacer length ranged from 19 to 27 nt showed similar base editing efficiencies at most of the editing positions. Frequencies were calculated from data in (a). Means ± s.d. were from three independent experiments.

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