Supplementary Figure 5: Features of dCpf1-BE-induced base editing

(a) Summary of the base editing frequency at each cytosine in the spacer region for the indicated 14 crRNAs. These data show that the major editing window ranges from the position 8 to 13 in spacer region. (b) The indel frequencies were determined at indicated loci in genomic DNA under different conditions. 293FT cells were either treated with dCpf1-BE (purple) or non-transfected (gray) before deep sequencing. Asterisk denotes an unusually high basal indel frequency (or amplification, sequencing, alignment artifact) at the examined RUNX1 site in the non-transfected 293FT cells. Means ± s.d. were from three independent experiments. (c) The fractions of cytosine substitutions induced by dCpf1-BE were individually determined at the indicated cytosines. (d) Statistical analysis showed that the C-to-T fraction of base editing outcome induced by dCpf1-BE was significantly higher than that induced by nCas9-BE3. The median and IQR are shown. P values, one-tailed Student’s T test. n = 42 independent samples from 3 independent experiments.