Supplementary Figure 7: Determination of base editing induced by dCpf1-BE at predicted off-target sites | Nature Biotechnology

Supplementary Figure 7: Determination of base editing induced by dCpf1-BE at predicted off-target sites

From: Base editing with a Cpf1–cytidine deaminase fusion

Supplementary Figure 7

(a) The sequences of on- and off-target sites for the indicated crRNAs. The cytosines were counted with the base proximal to the PAM setting as position 1. (b) The C-to-T editing frequencies of the indicated cytosines were individually determined at the indicated on- and off-target sites. 293FT cells were either treated with dCpf1-BE (purple) or non-transfected (gray) before deep sequencing. Means ± s.d. were from three independent experiments.

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