Supplementary Figure 5: Drug fitness replicate correlations with upstream and downstream synonymous codon changes for each SEC14 variant
From: Multiplexed precision genome editing with trackable genomic barcodes in yeast
(a) Log10 values of the normalized read counts are plotted for two replicate samples after 12 generations of growth in the presence of NPPM (n = 969 variants with read counts > 0 in at least one replicate). (b) The log2-fold change for each variant in the presence of NPPMs relative to the DMSO-control is plotted for the upstream synonymous changes version of each variant (y-axis) vs. the downstream synonymous changes version (x-axis). The Pearson correlation coefficient is indicated by pearsonr as in Supplementary Fig. 4b (n = 797 variants, filtered for amino acid variants with at least 10 reads for both upstream and downstream synonymous versions).
