Supplementary Figure 1: Barcode and feature representation throughout library construction

(a) Venn diagram representation of barcode (left) and feature (right) overlap among the oligo pool, step 1, and step 2 libraries. Note that barcodes are added during PCR amplification of the initial oligo library and therefore cannot be analyzed until cloning of step 1 libraries. Although most barcodes observed in step 1 libraries are not recovered in step 2 or yeast libraries, 88,821 out of 100,000 designed features are observed in the yeast pre-editing library (see Methods). (b) Representation of barcodes (left) and features (right) at the indicated stages of library construction as a function of subsampling reads from 0 to 20 million (left) or 10 million (right) reads. The barcodes/features are plotted as a percentage relative to all barcodes/features identified in their respective initial reference pools (step 1 cloning for barcodes and oligo pool for features). (c) Representation of barcodes (left) and features (right) at the indicated stages of library construction as a function of what percentage of the reads (Y) in each sample are attributed to what percentage (X) of the top barcodes/features in each sample, where the barcodes/features are sorted from highest to lowest abundance from left to right on the x-axis. The dotted line (slope = 1) depicts an idealized library of perfect uniformity where all members are present at equal abundance.