Supplementary Figure 10: Phenotypic validation of smORF mutants from heat and fluconazole screens.

Wildtype and selected sensitive and non-sensitive hits from smORF library screens are indicated. For the heat screen validation (a), cells were grown to log phase. 3μl of each undiluted and 5-fold serially diluted culture were spotted onto SC-URA plates that were incubated at the indicated temperatures for 48 hours and photographed. For the fluconazole screen validation (b), cells were cultured in a similar manner followed by spotting 5μl of each undiluted and 5-fold serially diluted culture onto SC-URA plates with and without fluconazole. Plates were incubated at 30ºC for 48 hours and photographed.