Supplementary Figure 1: Enhanced transformation efficiency with engineered guide+donor approach. | Nature Biotechnology

Supplementary Figure 1: Enhanced transformation efficiency with engineered guide+donor approach.

From: High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR–Cas9 in yeast

Supplementary Figure 1

a Schematic representations of unmodified guide+donor configuration (circular) and our engineered version (linearized) along with their corresponding transformation efficiencies. SNR52 promoter, N20, donor, and URA3 marker are labeled. Imaged plates are representative of 34 guide+donor yeast transformations in b targeting 34 different genomic sites performed with both configurations. b Scatterplot comparing transformation efficiencies of the selected 34 guide+donor plasmids between the unmodified and the engineered approaches in yeast strains expressing (blue) and not expressing Cas9 (orange). Genomic target corresponds to each guide+donor is indicated. Each guide+donor yeast transformation was performed twice. Relevant genotype of yeast strain background is exhibited on the x-axis. Average fold change in transformation efficiency is indicated on the y-axis and was calculated based on the number of transformants obtained from the engineered linearized plasmid setup divided by the number of transformants obtained from the circular plasmid transformation.

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