Supplementary Figure 3: Examining the influence of homology lengths on transformation efficiency of SNPs further away from the PAM. | Nature Biotechnology

Supplementary Figure 3: Examining the influence of homology lengths on transformation efficiency of SNPs further away from the PAM.

From: High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR–Cas9 in yeast

Supplementary Figure 3

a Schematic representation of a region of ADE2 centered around an N20-targeting site with SNPs (blue), SNPs distances, various homology lengths, cut site position, and wildtype and mutated PAMs (red) indicated. b Transformation efficiency of ADE2 guide+donors with varying donor homology lengths and SNPs distances from PAM as indicated on the legend. Transformation efficiency was calculated by the number of colonies obtained in the presence of Cas9 divided by the number of colonies in the absence of Cas9. Average transformation efficiency is displayed on the y-axis (n=2 independent cultures).

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